Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutchs pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nations first cancer prevention research program, as well as the clinical coordinating center of the Womens Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.
At Fred Hutch, we believe that the innovation, collaboration, and rigor that result from diversity and inclusion are critical to our mission of eliminating cancer and related diseases. We seek employeeswho bringdifferent and innovativeways of seeing the world and solving problems.Fred Hutch is in pursuit of becoming an antiracist organization.Weare committed to ensuring that all candidates hired share our commitment to diversity,antiracism, and inclusion.
This research opportunity is with the McElrath Lab within the Vaccine & Infectious Disease Division. The technician will work under the direction of a Staff Scientist and use discretion and judgment in performance of duties in support of human mucosal immunology studies relevant to HIV-1 prevention. In particular, they will develop and implement mucosal explant models and immunohistochemistry techniques for evaluating the functionality, levels and distribution of HIV-1 specific antibodies. They will also be responsible for processing various mucosal specimens from study participants, performing/developing downstream assays, collecting and analyzing the data. They may also be involved in COVID-19 related research, where similar laboratory techniques are utilized (e.g. ELISA). They will interpret and report on experimental results and assist with data presentation for conferences and manuscripts. They will interact closely with clinical staff (e.g. from the Fred Hutch HIV vaccine Trials Unit and/or other clinics) and laboratory staff to coordinate specimen collection and transport. They may also be responsible for transfer and processing of additional mucosal specimens from the University of Washington Medical Center (UWMC) and other hospitals. They will assist in the development and maintenance of laboratory quality control (QC) methods and records, which may require adherence to GCLP (Good Clinical Lab Practice) standards.
Coordinate specimen collection and transport with the Seattle VTU and laboratory staff
Coordinate sample shipping/receiving with collaborating laboratories
Responsible for the processing of cervicovaginal tissue specimens, and for the training of any laboratory staff who may assist with processing
Perform ex vivo infectivity assays on mucosal tissue, and train laboratory staff to be proficient in them
Perform luminescence reporter assays to measure HIV infection, and train laboratory staff to be proficient in them
Be available to process additional mucosal specimens as needed
Perform microdissection of mucosal tissue
Perform and develop immunohistochemistry assays on mucosal tissues
Perform confocal microscopy, including receptor and virus co-localization in mucosal tissue
Perform whole slide imaging microscopy
Perform and develop ELISAs, MSD and related assays to measure mucosal analytes (e.g. mucosal antibodies)
Perform and develop and/or optimize new assays and specimen processing methods as needed
Prepare single cell suspension from mucosal specimens, such as cytobrushes and cervicovaginal biopsies
May prepare and cryopreserve PBMC
May prepare stocks of HIV isolates by transient transfection
May perform and develop qPCR and/or digital droplet PCR assay (e.g. for detecting HIV-1 infection)
May perform immunophenotyping and/or functional profiling on single cell suspensions (e.g. by flow cytometry)
Help with production of manuscripts for new assays and studies by taking a significant role in writing, data analysis, making figures and interpretation of data
Identify methodological problems in research protocol and implement modifications to optimize experimental results
Prepare and present written or oral progress reports concerning studies including assembly, organization, and interpretation of data
Conduct literature reviews to assist in determining most suitable methods to be used in research
May perform statistical analyses or manage research database
Maintain records for experiments and quality control for the laboratory
Operate and carry out proper maintenance and QC of laboratory equipment
Maintain reagents, stocks, and cultures necessary to perform work
Order and maintain laboratory supplies
Other duties as assigned
Intermediate level position requiring a bachelors degree in a biological science and 2+ years of full-time, relevant experience performing and developing assays in a laboratory
Specific hands-on experience with cellular immunologic assays, tissue culture, immunohistochemistry and microscopy are required
Experience with micro-dissection, explant models, digital image analysis (e.g.HALO image analysis software) desirable
Motivation and adaptability are essential, as is a keen interest in vaccines and the prevention of infectious disease (especially HIV-1) fields
Must be willing to work with biohazardous/infectious agents (e.g., blood-borne pathogens, including HIV-1), including human tissues under carefully controlled conditions
Independent research capabilities and strong organizational and communication skills are necessary
Must be flexible and willing to work some evenings and weekends
A statement describing your commitment and contributions toward greater diversity, equity, inclusion, and antiracism in your career or that will be made through work at Fred Hutch is requested of all finalists.
Our Commitment to Diversity
We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at email@example.com or by calling 206-667-4700.
At Fred Hutchinson Cancer Research Center, teams of world-renowned scientists and humanitarians work together to prevent, diagnose and treat cancer, HIV/AIDS and other diseases. Researchers are discovering new ways to detect cancers earlier, improve treatments, and learn how to prevent cancers from growing. Although Fred Hutchinson opened its doors in 1975, its history began about 20 years before that. In 1962 Fred Hutchinson envisioned a center devoted to studying cancer, a disease that took his brother’s life. Today Fred Hutchinson is contributing to the next waves of breakthrough treatments and prevention strategies. Fred Hutchinson collaborates with the Seattle Cancer Alliance, the National Cancer Institute, and the University of Washington.