Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutchs pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nations first cancer prevention research program, as well as the clinical coordinating center of the Womens Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.
At Fred Hutch, we believe that the innovation, collaboration, and rigor that result from diversity and inclusion are critical to our mission of eliminating cancer and related diseases. We seek employees who bring different and innovative ways of seeing the world and solving problems. Fred Hutch is in pursuit of becoming an antiracist organization. We are committed to ensuring that all candidates hired share our commitment to diversity, antiracism, and inclusion.
This position will support projects to discover and characterize new antiviral factors. Lab tasks including CRISPR knock outs, PCR, DNA sequencing including next generation library preps, cell culture experiments, transformations, ligations, bacterial culture, minipreps, transfections, Western blot. Antiviral immune responses studied may include those that inhibit HIV, ZIKA and/or Coronaviruses. Data and samples must be well-organized and precisely tracked. The most critical qualities in a successful candidate will be organization, precision, and initiative. Under the direction and close supervision of the Principal Investigator or higher-level laboratory personnel in the lab, the applicant will gain practical experience in the following areas: - coordinating and performing support for a research laboratory that focuses on understanding innate immune responses to HIV and other viral infections
- performing state of the art CRISPR screening, in some cases in primary cell systems and validation of genes identified in such screens. Other basic molecular biology techniques will be developed as part of this work, including including learning the basics of molecular cloning, PCR, DNA sequencing, RT-PCR, flow cytometry, cell culture and transfection, and data analysis.
Responsibilities - Perform CRISPR KO screens and individual KOs
- Culturing of cell lines and primary T cells
- Prepare libraries for Illumina sequencing and analyze deep sequencing data
- Perform RNA seq
- Perform Western blots and other methods to measure gene/protein inactivation
- Perform molecular biology techniques such as PCR, RT-PCR, and ddPCR
- Assist with molecular cloning and tissue culture for a variety of projects, including studies of viral antibodies and the viral reservoir
- Maintain laboratory records and compile and verify the accuracy of data on experiments and assays
- Present experimental results in both oral and written report
- Perform other related duties as assigned
- Keep up to date on literature related to research projects
Qualifications Minimum qualifications: - BS required with extensive knowledge of cell and molecular biology
- Minimum of 2 years experience working in a laboratory
- Some scientific knowledge of virology and/or immunology is desirable
- Some experience with large data sets is desirable
- Must have sound laboratory skills, including molecular techniques and cell culture
- Must demonstrate exquisite organizational skills, laboratory precision, reliability and work initiative required to operate with minimal daily supervision
- Previous research experience in a BSL 2 laboratory, including tissue culture work is desirable
- Willingness to work in a HIV laboratory after suitable training (BSL2+)
Preference will be given to candidates with experience in the following areas: - Scientific knowledge of virology, immunology and/or RNA sequence analyses
- Previous laboratory experience (experiment design, execution, optimization, troubleshooting, data analysis)
- Demonstrated competence in careful and critical experimental design and execution.
- Lab management (inventory, ordering, equipment maintenance, etc.)
A statement describing your commitment and contributions toward greater diversity, equity, inclusion, and anti-racism in your career or that will be made through work at Fred Hutch is requested of all finalists. Our Commitment to Diversity We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at hrops@fredhutch.org or by calling 206-667-4700. |
