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The Johnston Laboratory is an interdisciplinary and highly collaborative team of classically trained microbiologists, synthetic microbiologists, bioinformaticians and engineers. Using cutting edge technologies and methodologies, we seek to gain a deeper functional understanding of what bacteria are doing and how they are doing it, and then to use that information to create novel therapeutics and engineer the next generation of microbe-based technologies for application in human medicine, synthetic microbiology and bioengineering.
The Johnston Laboratory in the Vaccine and Infectious Disease Division at Fred Hutch is recruiting a highly motivated and resourceful research technician with expertise in the genetic engineering/transformation of non-model bacterial species and general microbiology to join our cutting-edge microbial genomics efforts.
The position is intended to provide support for our ongoing microbial engineering projects and microbial technology developments, as well as supporting general laboratory responsibilities. The successful candidate will work with the PI and post-doctoral fellows using state-of-the-art approaches in synthetic microbiology and genomics in a highly collaborative, stimulating, and collegial research environment. The candidate will also assist in lab maintenance, including responsibility of ordering lab reagents/supplies and making of communal lab reagents. With interest and experience in our lab, the individual may assist with additional opportunities in research. This position will provide ample opportunities for professional development and the acquisition of cutting-edge skills in synthetic microbiology research, research planning and strategy, and data analysis and interpretation. The Johnston lab especially encourages those from backgrounds poorly represented in STEM fields to apply to the lab. Specific duties may include:
Aerobic and anaerobic bacterial culture and stock maintenance, genetic engineering/transformation of the such strains.
Adhere to standard operating procedures (SOPs) for isolation and analysis of DNA and RNA from microbial specimens, including PCR, quantitative PCR, RT-PCR, and sequencing.
Maintenance of general lab supplies, consumables and equipment
Conduct diligent record-keeping and reporting
Ordering/cataloging of laboratories supplies and reagents
Presenting findings at internal (Johnston Lab) meetings
Other research or organizational duties, as appropriate
Please include a cover letter with your application, detailing your interest and suitability for this position.
Bachelor's degree with 1-2 years experience in microbial research setting
Highly-motivated individual interested in a career in biological research or medicine, with a keen interest in genetic engineering of microbes
Technically meticulous when conducting experiments
Highly organized in record-keeping
Independent, intellectually passionate and able to display scientific initiative
Excellent teamwork, time management and organizational skills
Ability to work independently in a dynamic and results-oriented environment
Ability to meet deadlines and multitask efficiently is a must
Basic molecular biology and microbiology techniques, such as aseptic technique, bacterial isolation and culture, strain library maintenance, PCR, DNA/RNA/protein extraction, cloning, recombineering, etc. are stringently required.
A working knowledge and previous wet-lab experience with transformation of non-model bacterial organisms (optimization of competent cell preparation/heat shock/electroporation), in addition to standard coli transformations, is highly desirable and preferred.
Experience in the design and use of reagents for DNA cloning and genome engineering with shuttle vectors, suicide-vectors, CRISPR/Cas9 and transposon mutagenesis technologies also desirable.
Working knowledge and experience in anaerobic culture (anaerobic chamber use) will be beneficial to the role.
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At Fred Hutchinson Cancer Research Center, teams of world-renowned scientists and humanitarians work together to prevent, diagnose and treat cancer, HIV/AIDS and other diseases. Researchers are discovering new ways to detect cancers earlier, improve treatments, and learn how to prevent cancers from growing. Although Fred Hutchinson opened its doors in 1975, its history began about 20 years before that. In 1962 Fred Hutchinson envisioned a center devoted to studying cancer, a disease that took his brother’s life. Today Fred Hutchinson is contributing to the next waves of breakthrough treatments and prevention strategies. Fred Hutchinson collaborates with the Seattle Cancer Alliance, the National Cancer Institute, and the University of Washington.